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Resolution limit for DNA barcodes in the Odijk regime  
Resolution limit for DNA barcodes in the Odijk regime
资料类型: PDF文件
关键词: 蠕虫状链  排除体积效应  空间受限  微纳米通道  DNA条形码技术  
资料大小: 981k
所属学科: 高分子物理
来源: 来源网络
简介:
We develop an approximation for the probability of optically resolving two fluorescent labels on the backbone of a DNA molecule confined in a nanochannel in the Odijk regime as a function of the fluorescence wavelength, channel size, and the properties of the DNA (persistence length and effective width). The theoretical predictions agree well with equivalent data produced by Monte Carlo simulations of a touching wormlike bead model of DNA in a high ionic strength buffer. Although the theory is only strictly valid in the limit where the effective width of the nanochannel is small compared with the persistence length of the DNA, simulations indicate that the theoretical predictions are reasonably accurate for channel widths up to two-thirds of the persistence length. Our results quantify the conjecture that DNA barcoding has kilobase pair resolution-provided the nanochannel lies in the Odijk regime.
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上传时间: 2013-06-08 15:51:28
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